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3100 OFFGEL Fractionator

Protein fractionation, in-solution recovery

Research shows that prefractionation of proteins and peptides prior to LC/MS analysis can dramatically increase the number of proteins ultimately identified. The new Agilent 3100 OFFGEL Fractionator uses a novel isoelectric focusing technique to achieve excellent pI-based fractionation. The resulting fractions are in solution, making recovery for LC/MS analysis much easier than with old-fashioned gels. Results are highly reproducible, and the 3100 is a model of simplicity and ease of use.
  Agilent, Offgel Fractionator, 3100
 
Click here to see how the 3100 OFFGEL Fractionator works
 
  • pI-based OFFGEL fractionation with 0.1 – 0.6 pH resolution depending on consumable kit used
  • In-solution fraction recovery for easy transfer to LC/MS
  • Two power supplies allow simultaneous fractionation of two sample sets with broad differences in concentration
  • Up to 16 samples fractionated at the same time
  • µg to mg load capacity
  • Possible to run conventional in-gel IEF as well as OFFGEL fractionation
  • Preconfigured methods for OFFGEL and in-gel modes
  • Online current control for each individual sample
  • Simple, reproducible, and easy to use
 
Click here to see how the 3100 OFFGEL Fractionator quality of analysis
 
Click here to see how the 3100 OFFGEL Fractionator performance
 
 
Part Numbers and Prices
 
Description Part Number
   
Agilent 3100 OFFGEL Fractionator
Includes local controller, preinstalled software and default methods, power cable, two electrode sets, two trays, and optional installation and familiarization service.
G3100AA
Kits for OFFGEL electrophoresis mode
Starter Kit
Fractionation of protein or peptide samples.
Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples: three samples pH 3-10, 12 fractions; three samples pH 3-10, 24 fractions, three samples, pH 4-7, 12 fractions; three samples, pH 4-7, 24 fractions
5188-6444
Low Resolution Kit, pH 3-10
Fractionation of protein or peptide samples into 12 fractions in the pH range 3-10. Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples.
5188-6425
High Resolution Kit, pH 3-10
Fractionation of protein or peptide samples into 24 fractions in the pH range 3-10. Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples.
5188-6424
Low Resolution Kit, pH 4-7
Fractionation of protein or peptide samples into 12 fractions in the pH range 4-7. Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples.
5188-6427
High Resolution Kit, pH 4-7
Fractionation of protein or peptide samples into 24 fractions in the pH range 4-7. Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples.
5188-6426
OFFGEL Protein Test Sample
Checkout sample for testing the performance of the Agilent 3100 OFFGEL Fractionator. The checkout sample contains a single protein which will focus into only one fraction when used with the Low Resolution Kit, pH 3-10 (order no. 5188-6425).
5188-6428
Spare parts
Tray Set
Four replacement trays.
G3100-60007
Electrode Assembly
One tray, one fixed and one movable electrode.
G3100-60002
12-Well Frame Set
Set includes replacement parts for fractionation of 12 samples: well frames (12 wells), cover seals, electrode pads, glycerol solution, cover fluid and manual. Does not include IPG gel strips, ampholytes or reagents. To be used with 13-cm IPG gel strips and respective ampholytes. For use with 5188-6444, 5188-6425, 5188-6427.
5188-8012
24-Well Frame Set
Set includes replacement parts for fractionation of 12 samples: well frames (24 wells), cover seals, electrode pads, glycerol solution, cover fluid and manual. Does not include IPG gel strips, ampholytes or reagents. To be used with 24-cm IPG gel strips and respective ampholytes. For use with 5188-6444, 5188-6424, 5188-6426.
5188-8013

 

 

 


 
 
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