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Biochromatography - Glycoform and Glycan Analysis

Glycan Profiling for mAb Variant Characterisation. Glycans are oligosaccharides and polysaccharides widely distributed in biological systems in ‘free state’ and conjugated forms such as glycoproteins, glycolipids, and proteoglycans.

They are involved in a wide range of biological and physiological processes and are often key biomarkers for disease states such as cancer. Their functions, including efficacy and safety of protein-based drugs such as recombinant proteins and mAbs, are often dependent on the structure and types of glycans attached to the proteins.

The structures of glycans are quite diverse, complex, and heterogeneous. Glycosylation of antibodies is a prime source of product heterogeneity with respect to both structure and function. Variation in glycosylation is one of the main factors in product batch-to-batch variation, affecting product stability and Fc effector functions in vivo. As a result, the structural characterization and quantitative estimation of glycans is highly essential in biotherapeutics and biopharmaceutical projects.

 

Product Information

Crawford Scientific offers several specialty columns for glycan analysis. For more product information see the links below;

Agilent AdvanceBio Glycan Mapping columns

Thermo Scientific Glycanpac AXR-1 columns

 

 

Please contact us to discuss your specific biochromatography needs

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Application notes

Here at Crawford Scientific we have gathered all of the most relevant and useful documentation, including application notes, relating to glycan analysis, see below;

Agilent

Characterization of Glycosylation in the Fc Region of Therapeutic Recombinant Monoclonal Antibody

High Resolution Glycopeptide Mapping of EPO Using an Agilent AdvanceBio Peptide Mapping Column

Thermo

GlycanPac AXH-1 Column

GlycanPac AXR-1 Column

 
 
 

 

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