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Biochromatography - Ion Exchange Chromatography and Charge Variant Analysis

During production and purification, antibodies can exhibit changes in charge heterogeneity caused by amino acid substitutions, glycosylation, phosphorylation, and other post-translational or chemical modifications.

In protein analysis, charged variations at a given pH indicate a change in the primary molecular structure – resulting in additional forms of the protein in question. These are called isoforms (or charged variants), which can be resolved by IEX chromatography.

Because these changes can impact stability and activity – or cause immunologically adverse reactions – the analysis of charged variants is critical to biopharmaceuticals.



Product Information

Crawford Scientific can help you separate and characterise biomolecules with our family of ion-exchange BioHPLC columns. For more detailed product information click on the links below:

Agilent BioIEX column

Agilent BioMAb column

Thermo Scientific ProPac WCX-10 column

Thermo Scientific MAbPac SCX-10 column



Please contact us to discuss your specific biochromatography needs

contact analytical services

Application notes

Here at Crawford Scientific we have also gathered all of the most relevant and useful documentation, including application notes, relating to charge variant analysis using ion exchange, see below;


Charge Variant Analysis Applications

Rapid IgG1 Heavy Chain Analysis using Poroshell Columns

Anion Exchange Media – Protein Loading versus Resolution

Optimising Protein Separations using Weak Anion Exchange columns

pH gradient for Improved mAb Charge Variant Analysis

Fast Protein Separation using Weak Cation Exchange columns

Charge Variant Analysis using pH Gradients


DNAPac PA200 Column

mAb PAC columns

ProPac IEX

ProSwift Ion-Exchange Monolith Columns

ProSwift RP Monolith Protein Analysis



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