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Solid Phase Extraction or SPE is a separation technique that is rapidly growing in popularity and scope of use. Regardless whether your application is chemical, biological or environmental, you will see improved results using UltraPure SPE Cartridges. To reduce and in some cases eliminate the need to condition cartridges, which wastes your time and solvent removing extractables and fines, we use our UltraPure flash gel which has a low level of fines and is acid washed to remove all extractables.
Your needs are our top concern which is why we offer a wide variety of sorbents, and formats. All types of syringe barrels including flanged, flangeless and rimless and blocks are available. If you don't see the product you need please contact us. We can provide you with a custom solution for a standard price.
If you are new to SPE or working on a new protocol here is some information to help. SPE is a form of "digital chromatography", meaning it works in an on/off manner, compared to regular liquid chromatography where the elution on the peaks or components occurs gradually. SPE uses a sorbent that has a strong affinity for the desired compound. Solvent changes are used to collect the individual components as single fractions rather than gradient elution used in normal chromatography which yields multiple fractions of the same component. After applying the sample to a pre conditioned column the column is eluted with a solvent that has a weak affinity for the desired product to removed impurities it is then eluted with a solvent that has a strong affinity for the desired product thereby displacing it from the sorbent.
The process is shown visually in figure 1.
SPE was first used for the isolation of organic compounds from biological fluids such as blood and urine for toxicology and clinical work. It also become widely used for environmental research for the concentration and identification of trace organic pollutants. More recently organic and medicinal chemists have begun to utilize the technique to purify libraries of compounds. This is expanding the scope of sorbents to include solid supported scavengers and reagents.
Figure 2 and 3 are two tables designed to assist you in the selection of the sorbent. They are broken up by the solvent of the sample, aqueous (figure 2) and non-aqueous (figure 3) and subdivided by the properties of the desired compound which correspond to the recommended mechanism of interaction and sorbent type
Our standard syringe barrel format has a flanged or oblong rim. Flangeless, circular rim, and rimless are all available upon request.
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