ZORBAX 300Extend-C18 HPLC Columns

ZORBAX 300 Extend-C18 is a wide-bore HPLC column for high efficiency separations of peptides, polypeptides, and small proteins from pH 2 - 11.5. Extend-C18 and 300Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH . At high pH, the retention and selectivity of peptides can change dramatically as a result of changes in amino acid charge. Excellent recoveries of hydrophobic polypeptides have been achieved at high pH. In addition, LC/MS sensitivity of peptides can also be improved at high pH using a simple ammonium hydroxide mobile phase.