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in home > Products > Manufacturers > Agilent Technologies > HPLC Columns > ZORBAX GF-250/450 Size Exclusion Columns |
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ZORBAX GF-250/450 Size Exclusion Columns |
Ideal for the size separations of proteins and other biomolecules. The separation range is 4,000 - 900,000 for globular proteins when using a GF-250 and GF-450 column in series.
The GF-250/450size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and unique zirconia modification of the silica to extend the pH range from 3 - 8. The GF-250/450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions.
The result is a highly efficient, rugged and reproducible size exclusion column for separations of proteins with flow rates of up to 3 mL/min. These columns are compatible (typically 10% v/v or less) with organic modifiers and denaturants in the mobile phase to eliminate protein aggregation for proper size determination.
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- High efficiency and reproducibility with short analysis times
- Hydrophilic diol bonded phase for good protein recovery
- Compatible with organic modifiers and denaturants
- Wide usable pH range (pH 3 - 8)
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| Description |
Size (mm) |
ParticleSize (μm) |
Part Number |
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| ZORBAX GF-250 150Å |
9.4 x 250 |
4 |
884973-901 |
| ZORBAX GF-250 150Å |
4.6 x 250 |
4 |
884973-701 |
| ZORBAX GF-450 300Å |
9.4 x 250 |
6 |
884973-902 |
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| Columns Specifications |
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| Bonded Phase |
Pore Size |
Particle Size(µm) |
MW Range |
pH Range |
Surface Area |
Flow Rate |
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| ZORBAX GF-250 |
150Å |
4 |
4,000 - 400,000 |
3.0-8.0 |
140 m²/g |
<3.0 mL/min |
| ZORBAX GF-450 |
300Å |
6 |
10,000 - 900,000 |
3.0-8.0 |
50 m²/g |
<3.0 mL/min |
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