Q: What can I learn about chromatography or MS techniques in just one day?
A: The absolute essentials!
We have distilled critical knowledge of Chromatography and Mass Spectrometry applications and instrumentation into our range of exciting and interactive Short Courses. You will learn the absolute essentials of each technique and using our unique multi-media examples and tutorial exercises you will begin to use new knowledge and ideas right away.
These courses are invaluable for practicing analytical chemists who need a refresher, those new to analytical chemistry who require a solid grounding or for anyone wanting to gain an insight into important chromatographic and spectrometric techniques.
- We limit each course to 20 delegates so that individuals may participate in tutorial and interactive sessions
- Each delegate is supplied with a comprehensive delegate reference manual – not just a copy of the teaching slides used
- Each delegate is entitled to a reduced cost subscription to EARL – the Electronic Analytical Reference Library, the definitive multi-media reference tool for learning in analytical sciences
These Off-site short courses are held at prestigious venues throughout the UK.
More information – click here
Register – click here
Fundamentals of High Performance Liquid Chromatography (HPLC) |
- The basis of instrument hardware design
- Modes of chromatographic separation – Reverse phase %B / ion pair reagents / optimising separations etc.
- Inter-relationship of resolution with other chromatographic descriptors
- Introduction to basic troubleshooting and maintenance
- Overview of current column chemistry
……and more! BOOK NOW
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HPLC Troubleshooting & Maintenance |
- Common hardware problems associated with: mobile phase preparation, injector, pump, column and detector
- Troubleshooting the chromatogram through an evaluation of;
- The separation chemistry - pH and buffer strength effects
- Changes to the chromatographic baseline – cyclical, fluctuating etc.
- Changes to chromatographic peak shape – tailing, splitting etc.
- Changes in the HPLC system operating pressure and analyte retention times and peak areas
……and more! BOOK NOW
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LC−MS for the Chromatographer |
- An overview of instrument hardware design
- Electrospray (ESI) and Atmospheric Pressure chemical Ionisation (APcI) interfaces
- ESI analyte gas phase ion production and optimisation
- APcI analyte gas phase ion production and optimisation
- Single and tandem MS data acquisition modes and elements of interpretation
- A comprehensive set of application specific compound datasheets
- Interactive worked examples and course tutorial questions
……and more! BOOK NOW
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Interpretation of LC−MS Data |
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- Understanding isotope patterns and relative responses for confirmation of:
- Elemental composition and chemical structure
- Formation of common adduct ions under different mobile phase conditions
- Compound dimerisation, multiply charged ions and the deconvolution process
- Precursor ion collisionally induced dissociation (CID) fragmentation including:
- Structural elucidation − The alpha-cleavage and inductive-cleavage
- Target ion characterisation, peptide sequencing etc.
- MS/MS scan function experiments − when to use them and how to interpret the results
- Interpretation practice through consultant led tutorial exercises
……and more! BOOK NOW
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Fundamentals of Gas Chromatography (GC) |
- The basis of instrument hardware design
- Sample preparation and the basics of the chromatographic process
- Inlet systems, column and detector selection
- Modes of chromatographic separation
- Introduction to basic troubleshooting and maintenance
……and more! BOOK NOW
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GC Troubleshooting & Maintenance |
- The inlet system, column and detector
- Troubleshooting the chromatogram is investigated through an evaluation of;
- Inlet system optimisation – split/splitless, temperature, deactivation etc.
- Detector optimisation
- Changes to the chromatographic baseline – drifting, noisy etc.
- Changes to chromatographic peak shape – broadening, splitting etc.
……and more! BOOK NOW |
GC-MS for the Chromatographer
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- An overview of instrument hardware design
- Electron Impact (EI) and Chemical Ionisation (CI) interfaces
- Analyte EI gas phase ion production and optimisation
- Analyte CI gas phase ion production and optimisation
- Principles of mass analyser design and experiment choices
- Single and Full Scan MS data acquisition modes and elements of interpretation
- A comprehensive set of application specific compound datasheets
- Interactive worked examples and course tutorial questions
……and more! BOOK NOW
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Interpretation of GC−MS Data |
- Review of the chemistry of ions, electrons, radicals and isotopes
- Principles of Electron Impact (EI) and Chemically Induced (CI) ionisation mechanisms
- Fragmentation mechanisms including:
- Homo and heterolytic fission, alpha-cleavage, McLafferty rearrangement etc. Interpretation rules including:
- Isotopic abundances and normalisation, the Nitrogen rule, rings and double bonds etc.
- Interpretation practice through consultant led tutorial exercises
……and more! BOOK NOW
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