Chromatography Consumables, Analytical Services and Chromatography / Mass Spectrometry Training from Crawford Scientific
 
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Gas Chromatography - Mass Spectrometry



 GC-MS for the Chromatographer
GC-MS Interpretation

Each of our GC-MS courses are supplemented with full colour delegate handbooks as well as tutorial exercises and interactive multi-media from our EARL product range.

Each course may be customised to you specific business and analytical requirements.

Each of these courses can be assessed if required

For more information on these courses or to download our 2008 training brochure – click here
 

 GC-MS for the Chromatographer

This course is designed to cover the basic operating and theoretical principles of gas chromatography using mass selective detection. It will be most useful to those who have a working knowledge of GC-MS but wish to understand the principles that govern the technique.


 Chromatographic Considerations


  • Basic Principles of the Chromatographic Process - Partition Theory
  • Mechanisms of Gas Chromatography
  • the equilibrium / distribution constant & solute-stationary phase interactions
  • Temperature / Retention Relationship
  • Sample Introduction principles and description of split / splitless, large volume (PTV) and on-column injection systems.
  • Particular attention will be paid to carrier flow and sample loading
 


 GC- MS Hardware

  • Low bleed phases and column geometries' compatible with mass selective detection
  • The Mass Selective Detector
  • System Hardware - description and operational principles (system overview, transfer line, pumping systems, ion source, quadrupole mass analyser, ion trap (where appropriate), electron multipliers and detector electronics)
  • Why is Vacuum Required? - including monitoring and maintaining vacuum systems
  • The Ion Source - why is ionisation necessary?
  • Operating principle of the ion source, consideration of ion formation / fragmentation, beam collimation and acceleration.
  • Solvent vent time and ion source maintenance
  • Quadrupole Mass Filters - construction and operating principles
  • Electron Multiplier principle of operation as both detector and amplifier.


 Tuning, Data Acquisition and Sample Interpretation


  • Flow Chart of a Typical Auto-Tune Routine, tuning standards, relative abundance, isotope ratios, mass assignments and peak width.
  • Detector Electronics Mathieu diagrams used to explain the significance of AMU gain and offset, X-ray voltage, electron multiplier voltage
  • Troubleshooting from the Auto Tune Report how to identify system problems such as a dirty ion source
  • Air and Water Checks
  • Full Scan and Selected Ion Detection Modes principles of each mode, sensitivity enhancement, and scan rate settings
  • Common Mass Spectral Features some common fragmentation and isotopic patterns


 GC- MS Interpretation

This very popular course covers the principles of how to obtain good spectra – as well as having a host of tutor led exercises for crucial practice of interpretation skills and techniques.
  • Review of the chemistry of ions, electrons, radicals and isotopes
  • Principles of Electron Impact (EI) and Chemically Induced (CI) ionisation mechanisms
  • Fragmentation mechanisms including:
  • Homo and heterolytic fission, alpha-cleavage, McLafferty rearrangement etc. Interpretation rules including:
  • Isotopic abundances and normalisation, the Nitrogen rule, rings and double bonds etc.
  • Interpretation practice through consultant led tutorial exercises
  • ……and more!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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